In March 2022, the ACLU, ACLU of Minnesota, and ACLU of Southern California filed a lawsuit on behalf of three Muslim Americans who have been subjected to intrusive questioning from border officers about their religious beliefs, practices, and associations in violation of their constitutional rights.

The American Civil Liberties Union, the ACLU of Minnesota, and Stinson Leonard Street LLP filed a motion to intervene on behalf of a transgender student in a lawsuit that seeks to bar trans students from using locker rooms consistent with their gender identity. This case is similar to lawsuits filed around the country that are trying to prevent transgender students from using the locker rooms and restrooms that match their gender identity.